Journal: Computational and Mathematical Methods in Medicine

Article Title: Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

doi: 10.1155/2021/5005459

Figure Lengend Snippet: ASF1B promotes the proliferation of LUAD. (a, d) Colony formation assays, (b) CCK-8 assays depict that knockdown of ASF1B inhibits proliferation of LUAD cells. (c) Western blot analysis to evaluate the levels of proliferation signaling associated proteins' expression levels in LUAD.

Article Snippet: Non-neoplastic lung epithelial cell lines (HBE) and six LUAD cell lines (A549, H460, H1299, H1650, H1975, and PC9) were obtained from ATCC (American Type Culture Collection).

Techniques: CCK-8 Assay, Knockdown, Western Blot, Expressing

Journal: Computational and Mathematical Methods in Medicine

Article Title: Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

doi: 10.1155/2021/5005459

Figure Lengend Snippet: ASF1B promotes proliferation of LUAD via regulating BCAR1. (a, b) Colony formation assays. (c, d) CCK8 assays indicating induced BCAR1 in ASF1B knockdown A549 and PC9 cells restored cell proliferation ability. (e) Restoration of BCAR1 upregulated CDK2/4 and CCND1 and decreased P27 in ASF1B knockdown cells.

Article Snippet: Non-neoplastic lung epithelial cell lines (HBE) and six LUAD cell lines (A549, H460, H1299, H1650, H1975, and PC9) were obtained from ATCC (American Type Culture Collection).

Techniques: Knockdown

Journal: Computational and Mathematical Methods in Medicine

Article Title: Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

doi: 10.1155/2021/5005459

Figure Lengend Snippet: ASF1B promotes migration of LUAD cells by promoting BCAR1. (a, d) Wound healing. (b, c) Transwell assays depict increased migration ability after overexpressing BCAR1 in ASF1B knockdown LUAD cells. (e) Western blot results demonstrate decreased N-cadherin, Slug, Snail, Vimentin, and increased E-cadherin in ASF1B knockdown A549 and PC9 cells with BCAR1 overexpressed.

Article Snippet: Non-neoplastic lung epithelial cell lines (HBE) and six LUAD cell lines (A549, H460, H1299, H1650, H1975, and PC9) were obtained from ATCC (American Type Culture Collection).

Techniques: Migration, Knockdown, Western Blot

Journal: Computational and Mathematical Methods in Medicine

Article Title: Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses

doi: 10.1155/2021/5005459

Figure Lengend Snippet: Correlation of ASF1B with 64 types of noncancerous cell populations in LUAD.

Article Snippet: Non-neoplastic lung epithelial cell lines (HBE) and six LUAD cell lines (A549, H460, H1299, H1650, H1975, and PC9) were obtained from ATCC (American Type Culture Collection).

Techniques: Clinical Proteomics

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: KIS, a target of SOX4, regulates the ID1-mediated enhancement of β-catenin to facilitate lung adenocarcinoma cell proliferation and metastasis

doi: 10.1007/s00432-024-05853-9

Figure Lengend Snippet: KIS is highly expressed in LUAD tissues and LUAD cell lines. (A) KIS mRNA expression in LUAD tissues and normal tissues was analyzed using the UALCAN website based on TCGA database. (B-D) The mRNA levels of KIS, ID1 and SOX4 were detected in LUAD tissues from patients who did not receive chemoradiotherapy and paracancerous samples using reverse transcription-quantitative PCR. (E) The protein levels of KIS were detected LUAD tissues from patients who did not receive chemoradiotherapy and paracancerous samples using western blot analysis (part of the data). ** P < 0.01, compared with normal tissues or paracancerous samples. KIS, Kinase interacting with stathmin; LUAD, lung adenocarcinoma; TCGA, The Cancer Genome Atlas; ID1, inhibitor of DNA binding 1; SOX4, (Sry)-box-containing 4

Article Snippet: A total of six LUAD cell lines (cat. no. iCell-h011, A549; cat. no. iCell-h155, NCI-H1650; cat. no. iCell-h156, NCI-H1975; cat. no. iCell-h068, HCC-827; cat. no. iCell-h159, NCI-H358 and cat. no. iCell-h153, NCI-H1299) and an immortalized normal human bronchial epithelial cell line (cat. no. iCell-h023, BEAS-2B) were purchased from iCell Bioscience Inc. and STR profiling was used for authentication.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: KIS, a target of SOX4, regulates the ID1-mediated enhancement of β-catenin to facilitate lung adenocarcinoma cell proliferation and metastasis

doi: 10.1007/s00432-024-05853-9

Figure Lengend Snippet: KIS facilitates LUAD development by modulating ID1. (A) The transfection efficiency of ID1 shRNA in NCI-H1650 cells was verified at the mRNA and protein level. KIS-overexpressing NCI-H1650 cells were transfected with ID1 siRNA to silence ID1, and these cells were subjected to analyses following 24 h of transfection. (B) Cell Counting Kit-8 assay of cellular proliferation. (C) The migratory (upper panel) and invasive (lower panel) capacities of cells were evaluated at 24 h following transfection using Transwell assay (scale bar, 100 μm). (D) The numbers of migratory and invasive cells were quantified. (E) The protein levels of PCNA, CDK4, MMP-9 and MMP-2 were detected using western blot analysis. (F) TOP/FOP flash assay revealed the luciferase activity of β-catenin. (G) The mRNA levels of c-Myc and Axin2 were measured. * P < 0.05 and ** P < 0.01, compared with shNC group, vector group or KIS + si-NC group. KIS, kinase interacting with stathmin; ID1, inhibitor of DNA binding 1; Axin2, axis inhibition protein 2; PCNA, proliferating cell nuclear antigen; CDK4, cyclin-dependent kinase 4; MMP, matrix metalloproteinase

Article Snippet: A total of six LUAD cell lines (cat. no. iCell-h011, A549; cat. no. iCell-h155, NCI-H1650; cat. no. iCell-h156, NCI-H1975; cat. no. iCell-h068, HCC-827; cat. no. iCell-h159, NCI-H358 and cat. no. iCell-h153, NCI-H1299) and an immortalized normal human bronchial epithelial cell line (cat. no. iCell-h023, BEAS-2B) were purchased from iCell Bioscience Inc. and STR profiling was used for authentication.

Techniques: Transfection, shRNA, Cell Counting, Transwell Assay, Western Blot, Luciferase, Activity Assay, Plasmid Preparation, Binding Assay, Inhibition

Journal: Bioengineered

Article Title: N6-methyladenosine-induced SVIL antisense RNA 1 restrains lung adenocarcinoma cell proliferation by destabilizing E2F1

doi: 10.1080/21655979.2022.2025697

Figure Lengend Snippet: SVIL-AS1 overexpression leads to the decreased cell proliferation and cell cycle arrest of LUAD. (a) The relative expression of SVIL-AS1 was determined in SVIL-AS1-knockdown and forced-expressing cells by qRT-PCR in A549 and H1650 cells. (b–d) CCK-8 assay (b), BrdU staining (c), and cell cycle analysis (d) were performed in A549 and H1650 cells overexpressing SVIL-AS1. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: LUAD cells (A549 and H1650) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Over Expression, Expressing, Knockdown, Quantitative RT-PCR, CCK-8 Assay, BrdU Staining, Cell Cycle Assay

Journal: Bioengineered

Article Title: N6-methyladenosine-induced SVIL antisense RNA 1 restrains lung adenocarcinoma cell proliferation by destabilizing E2F1

doi: 10.1080/21655979.2022.2025697

Figure Lengend Snippet: METTL3-mediated SVIL-AS1 suppresses E2F1 and E2F1-target genes in LUAD cells. (a,b) A549 and H1650 cells were transfected with shSVIL-AS1 #1, shSVIL-AS1 #2 or shscr. The relative expression of E2F1, CCNE1, MYBL2, CDC25A, p27 and p15 was determined by qRT-PCR. (c,d) A549 and H1650 cells were cotransfected with shMETTL3 and/or SVIL-AS1. he relative expression of E2F1, CCNE1, MYBL2, CDC25A, p27 and p15 was determined by qRT-PCR. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: LUAD cells (A549 and H1650) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Transfection, Expressing, Quantitative RT-PCR

Journal: Bioengineered

Article Title: N6-methyladenosine-induced SVIL antisense RNA 1 restrains lung adenocarcinoma cell proliferation by destabilizing E2F1

doi: 10.1080/21655979.2022.2025697

Figure Lengend Snippet: METTL3 regulated SVIL-AS1 has an inhibitory effects on cell proliferation of LUAD by E2F1. (a) CCK-8 assay (b) BrdU staining and (c) cell cycle analysis were performed to detect cell viability, cell proliferation, and cell cycle transition. A549 and H1650 cells were co-transfected with SVIL-AS1 overexpression, E2F1 overexpression, or shMETTL3 overexpression. * p < 0.05, ** p < 0.01, *** p < 0.001. (d,e) The relative expression of E2F1 and METTL3 was detected by qPCR and Western blotting in A549 and H1650 cells co-transfected with SVIL-AS1 overexpression plus E2F1 overexpression or shMETTL3. The relative expression of SVIL-AS1 was measured by qPCR. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: LUAD cells (A549 and H1650) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: CCK-8 Assay, BrdU Staining, Cell Cycle Assay, Transfection, Over Expression, Expressing, Western Blot

Journal: Bioengineered

Article Title: N6-methyladenosine-induced SVIL antisense RNA 1 restrains lung adenocarcinoma cell proliferation by destabilizing E2F1

doi: 10.1080/21655979.2022.2025697

Figure Lengend Snippet: SVIL-AS1 reduced tumor growth in vivo . (a) A549 cells stably expressing SVIL-AS1, SVIL-AS1 plus E2F1 (SVIL-AS1 +E2F1), SVIL-AS1 plus METTL3 knockdown (SVIL-AS1+shMETTL3), and vector plus sh-NC (negative control, NC). Images of xenograft tumors in each group are shown (n = 5). (b) Tumor volumes were measured every 4 days, and growth curves were drawn. * p < 0.05, ** p < 0.01, *** p < 0.001. (c) Tumor weight was calculated for each group. ** p < 0.01. (d) Schematic illustration of the mechanism SVIL-AS1 regulated by METTL3 to suppress LUAD tumourigenesis by targeting E2F1 and its target genes.

Article Snippet: LUAD cells (A549 and H1650) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: In Vivo, Stable Transfection, Expressing, Knockdown, Plasmid Preparation, Negative Control